bersempena tamatnya sudah annual scientific meeting utk SSM ni, ingin ana kongsikan gmbr2 group kami sewaktu organise and implement data collection huhu...so, let me tell you according to the photo...
firstly, purchasing the male Wistar r ats from the LARU...we bought about 83 rats for our study and let them in our lab for about 3 days to a weeks for them to 'membiasakan diri' in a new place...then, before we take their blood, we need to fast them for 12 hours...but we didi it for almost 17 hours ( 4pm till 9am tomorrow morning)....
then, come to the session of blood taking...actually we anaesthatis first the rats and we take the blood from the medial canthus..it is not an easy procedure because as a medical student we are teach to take blood from the vein not from the canthus (eye)...
then, we let the rats to regained consciousness for a while before we restrained the rats' tail on the board to amputate their rats and do the bleeding time and clotting time count...
this is ehsan and my speciality as taking blood is chai peng's speaciality...huhu...lama nak kene tgu...average 5-10 min per rate for this procedure...bayangkanla ada sehari 2 kami habiskn 40 ekor terus...mmg xtercapai dek akal mula2...pas2 barulah mula treatment....treatment 2 ap??
sebenarnya bukan treatment utk penyakit...tp kami nk cb bg tikus2 ni mkn ulam raja tp high dose punya...actually accordinglt la...ikut OECD 2004; fixed dose method....we fed them with ulam raja...haa jgn terkejut...bkn org je mkn ulam raja...tikus pun makan....kami bg force feeding pkai oral gavage yg trs msk smpai stomach...supaya tikus2 2 xla rasa pahit kt tekak...huhu...ini plk derick and epa speciality....
we treat them 1 day for acute study and 14 days for subacute study. then, we do the same procedure again, taking blood, do the clotting and bleeding time count and lastly we let the rat sleep in peace forever before we take their organ and weight them...
and this time i bwk aishah humaira' together bcoz xd sp nk jaga...babysitter dia demam...so bermain2la aishah didlm lab....
sedang umminya membuat keje2 menimbang organ tikus, aishah yg kepenatan mintak perhatian...merengek2....pening jap + penat...(berdiri all the time mcm keje kilang...huhu)
then, dptla perhatian si ummi..berdukunglah si anak...tanpa protection mcm ummi dia...huhu...
last sekali bila ummi dh hbs keje, time to enjoy...best sgt masuk universiti ni...esok2 dh besar aishah nk msk U jgkla ummi...ok sayang....
sampai sini dulu, t ummi post lagi ya...daa~
salam sayang,
ummuhumaira'
1 comments:
it is funny that white coat can do such thing. cooking is it ?
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